Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 15, Pages 8050-8056Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01494
Keywords
-
Categories
Funding
- Science and Technology Ministry of China [2016YFA0201200]
- National Natural Science Foundation of China [21327902, 21535003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
We report a multicolor ECL device based on closed bipolar electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)(3)](2+) and [Ir(ppy)(3)] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green-yellow-red ECL emission changing. As the first multicolor ECL device in biological analysis, BPE may raise the application of potential-resolved ECL to a new level.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available