Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 2, Pages 1257-1270Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.001257
Keywords
-
Funding
- European Research Council (ERC) [StG 279753]
Ask authors/readers for more resources
The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available