Journal
BIOMEDICAL OPTICS EXPRESS
Volume 9, Issue 1, Pages 25-40Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.9.000025
Keywords
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Funding
- Boston Medical Center
- NIH [5R01CA183101]
- BU-CTSI pilot grant [1UL1TR001430]
- BU-Joslin pilot program (BU-CTSI) [KL2TR001411]
- Bright focus foundation
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While fluorescent contrast is widely used in ophthalmology, three-dimensional (3D) fluorescence retinal imaging over a large field of view (FOV) has been challenging. In this paper, we describe a novel oblique scanning laser ophthalmoscopy (oSLO) technique that provides 3D volumetric fluorescence retinal imaging with only one raster scan. The technique utilizes scanned oblique illumination and angled detection to obtain fluorescent cross-sectional images, analogous to optical coherence tomography (OCT) line scans (or B-scans). By breaking the coaxial optical alignment used in conventional retinal imaging modalities, depth resolution is drastically improved. To demonstrate the capability of oSLO, we have performed in vivo volumetric fluorescein angiography (FA) of the rat retina with similar to 25 mu m depth resolution and over a 30 degrees FOV. Using depth segmentation, oSLO can obtain high contrast images of the microvasculature down to single capillaries in 3D. The multi-modal nature of oSLO also allows for seamless combination with simultaneous OCT angiography. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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