Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 12, Pages 5412-5426Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.005412
Keywords
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Funding
- NSF NCS-FO grant [CBET 1631704]
- National Science Foundation (NSF) IDBR grant [DBI-1353757]
- Office of Naval Research (ONR) [N00014-15-1-2739]
- National Institute of Health (NIH) SPARC grant [OT2OD023852]
- University of Colorado Boulder Libraries Open Access Fund
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1353757] Funding Source: National Science Foundation
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Laser scanners are an integral part of high resolution biomedical imaging systems such as confocal or 2-photon excitation (2PE) microscopes. In this work, we demonstrate the utility of electrowetting on dielectric (EWOD) prisms as a lateral laser-scanning element integrated in a conventional 2PE microscope. To the best of our knowledge, this is the first such demonstration for EWOD prisms. EWOD devices provide a transmissive, low power consuming, and compact alternative to conventional adaptive optics, and hence this technology has tremendous potential. We demonstrate 2PE microscope imaging of cultured mouse hippocampal neurons with a FOV of 130 x 130 mu m(2 )using EWOD prism scanning. In addition, we show simulations of the optical system with the EWOD prism, to evaluate the effect of propagating a Gaussian beam through the EWOD prism on the imaging quality. Based on the simulation results a beam size of 0.91 mm full width half max was chosen to conduct the imaging experiments, resulting in a numerical aperture of 0.17 of the imaging system. (C) 2017 Optical Society of America under the terms of the of the OSA Open Access Publishing Agreement
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