Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 6, Pages 3110-3118Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.003110
Keywords
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Funding
- Human Frontiers Scientific Program [RGP0004/2013]
- Red Cardiovascular (RIC) ISCIII [RD12/0042/0057]
- Ministerio de Economia y Competitividad
- Centro de Investigacion Biomedica en Red de Salud Mental
- EC FP7 CIG grant HIGH THROUGHPUT TOMO
- BRADE [S2013/ICE-2958]
- Fondos FEDER Una manera de hacer Europa
- MINECO [FIS2016-77892-R]
- Pro-CNIC Foundation
- Severo Ochoa Center of Excellence (MINECO) [SEV-2015-0505]
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The ability to acquire 3D images of the heart and its vasculature at cellular resolution facilitates a more detailed study of many heart diseases. Here, we describe a novel technique to image in 3D the heart vasculature by combining the CUBIC clearing protocol combined with in vivo administration of fluorescent-labeled lectin. The use of these techniques in combination with Selective Plane Illumination Microscopy (SPIM) made it possible to obtain high resolution 3D images of the cardiac vascular tree. This methodological approach may enhance the visualization of 3D images of the cardiac vasculature remodeling associated with coronary disease. (C) 2017 Optical Society of America
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