Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 11, Pages 5127-5138Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.005127
Keywords
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Funding
- Australian Research Council (Australia)
- National Health and Medical Research Council (Australia)
- National Breast Cancer Foundation (Australia)
- Department of Health, Western Australia
- Cancer Council, Western Australia
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In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens micro-endoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1 : 6 mu m and an axial resolution of 2 : 2 mu m. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 mu m, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo. (C) 2017 Optical Society of America.
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