Surfactant-induced enhancement of droplet adhesion in superhydrophobic soybean (Glycine max L.) leaves

This study performed with soybean (Glycine max L.), one of the most important crops for human and animal nutrition, demonstrates that changes in the leaf surface structure can increase the adhesion of applied droplets, even on superhydrophobic leaves, to reduce undesirable soil contamination by roll-off of agrochemical formulations from the plant surfaces. The wettability and morphology of soybean (Glycine max L.) leaf surfaces before and after treatment with six different surfactants (Agnique (R) SBO10 and five variations of nonionic surfactants) have been investigated. The leaf surface structures show a hierarchical organization, built up by convex epidermal cells (microstructure) and superimposed epicuticular platelet-shaped wax crystals (micro-to nanostructure). Chemical analysis of the epicuticular wax showed that 1-triacontanol (C30H61OH) is the main wax component of the soybean leaf surfaces. A water contact angle (CA) of 162.4 degrees (sigma = 3.6 degrees) and tilting angle (TA) of 20.9 degrees (sigma = 10.0 degrees) were found. Adherence of pure water droplets on the superhydrophobic leaves is supported by the hydrophilic hairs on the leaves. Agnique (R) SBO10 and the nonionic surfactant XP ED 75 increased the droplet adhesion and caused an increase of the TA from 20.9 degrees to 85 degrees and 90 degrees, respectively. Scanning electron microscopy showed that surfactants with a hydrophilic-lipophilic balance value below 10 caused a size reduction of the epicuticular wax structures and a change from Cassie-Baxter wetting to an intermediate wetting regime with an increase of droplet adhesion.