Journal
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
Volume 8, Issue -, Pages 1396-1406Publisher
BEILSTEIN-INSTITUT
DOI: 10.3762/bjnano.8.141
Keywords
Caco-2; differentiation and polarisation; epithelial cell barrier; microscopy imaging; particle interaction; uptake and localisation
Funding
- INSPIRE (Integrated NanoScience Platform for IREland) programme - Irish Government's Programme for Research in Third Level Institutions, Cycle 4, National Development Plan
- Irish Research Council Enterprise Partership Postdoctoral Scheme
- EU FP7 [NMP4-SL-2008-214547, NMP4-2010-EU-US-266737]
- Science Foundation Ireland [09/RFP/MTR2425]
- Science Foundation Ireland (SFI) [09/RFP/MTR2425] Funding Source: Science Foundation Ireland (SFI)
Ask authors/readers for more resources
Cellular barriers, such as the skin, the lung epithelium or the intestinal epithelium, constitute one of the first obstacles facing nanomedicines or other nanoparticles entering organisms. It is thus important to assess the capacity of nanoparticles to enter and transport across such barriers. In this work, Caco-2 intestinal epithelial cells were used as a well-established model for the intestinal barrier, and the uptake, trafficking and translocation of model silica nanoparticles of different sizes were investigated using a combination of imaging, flow cytometry and transport studies. Compared to typical observations in standard cell lines commonly used for in vitro studies, silica nanoparticle uptake into well-developed Caco-2 cellular barriers was found to be very low. Instead, nanoparticle association to the apical outer membrane was substantial and these particles could easily be misinterpreted as internalised in the absence of imaging. Passage of nanoparticles through the barrier was very limited, suggesting that the low amount of internalised nanoparticles was due to reduced uptake into cells, rather than a considerable transport through them.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available