Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 30, Pages 25128-25137Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b06404
Keywords
nanosensor; nitric oxide sensing; osteoarthritis; DAF-FM; PLGA
Funding
- NTU-Northwestern Institute for Nanomedicine
- LKC Medicine-SCBE Collaborative Grant
- National key research and development program of China [2016YFB0700804]
- Guangxi Science Fund for Distinguished Young Scholars [2014GXNSFGA118006]
- Guangxi Scientific Research and Technological Development Foundation [GuikeAB16450003]
- High level innovation teams and outstanding scholars in Guangxi Universities (the third batch)
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Osteoarthritis (OA) is a chronic arthritic disease that causes the overproduction of inflammatory factors such as nitric oxide (NO). This study develops a NO nanosensor to predict the OA development. The nanosensor is synthesized by encapsulating the NO sensing molecules (i.e., 4-amino-5-methylamino-2',7'-difluorofluorescein Diaminofluorescein-FM (DAF-FM)) within the biodegradable poly (lactic-co-glycolic acid) nanoparticles. In vitro, the nanosensor allows the monitoring of the NO release in interleukin-1 beta-stimulated chondrocytes and the alleviated effect of N-G-monomethyl-L-arginine (a NO inhibitor) and andrographolide (an anti-inflammatory agent). In the rat OA model, it permits the quantification of NO level in joint fluid. The proposed NO nanosensor may facilitate a noninvasive and real-time evaluation of the OA development.
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