Journal
ACTA CHIMICA SLOVENICA
Volume 64, Issue 2, Pages 491-499Publisher
SLOVENSKO KEMIJSKO DRUSTVO
DOI: 10.17344/acsi.2017.3408
Keywords
Chromogenic radicals; antiradical activity; Folin-Ciocalteu; reaction kinetics; Trolox equivalents; solvent composition
Categories
Funding
- Slovenian Research Agency [P4-0121]
Ask authors/readers for more resources
Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined for coffee, tea, beer, apple juice and dietary supplements. Large differences between the AOP values depending on the reaction medium were observed, with the highest AOP determined mostly in the FC assay. In reactions with chromogenic radicals several fold higher values of AOP were obtained in buffer pH 7.4 than in water or methanol. The type of assay and solvent composition have similar influences on the reactivity of a particular antioxidant, either pure or as part of a complex matrix. The reaction kinetics of radicals with antioxidants in samples reveals that AOP depends strongly on incubation time, yet differently for each sample analyzed and the assay applied.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available