4.5 Article

Simple and flexible phase compensation for digital holographic microscopy with electrically tunable lens

Journal

APPLIED OPTICS
Volume 56, Issue 21, Pages 6007-6014

Publisher

OPTICAL SOC AMER
DOI: 10.1364/AO.56.006007

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Funding

  1. National Natural Science Foundation of China (NSFC) [61171073, 61307003, 61377017]
  2. Sino-German Center for Research Promotion [GZ 760]
  3. Natural Science Foundation of SZU [2016028]
  4. Science and Technology Innovation Commission of Shenzhen [JCYJ20160520164642478]

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In a digital holographic microscopy (DHM) system, different microscope objectives (MOs) will introduce different phase distortions and thus lead to measurement errors. To address this problem, we present a simple and flexible method to compensate all phase distortions by introducing an electrically tunable lens (ETL) in the reference arm for a DHM system with multiple MOs. By exactly controlling the external currents of the ETL, we can change the reference wave front to match the wave front introduced by different MOs without complex alignment or additional numerical postprocessing manipulations. This method is suitable for quantitative real-time phase imaging especially when it refers to multiple MOs. To demonstrate the validity and effectiveness of our scheme, we did a series of simulations and carried out some real experiments with two different MOs (4x and 10x). (C) 2017 Optical Society of America

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