Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-017-01221-z
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Funding
- National Health and Medical Research Council [APP1058442, APP1045677, APP1041582, APP1023460, APP1005030, APP1043978]
- Australian Research Council
- Cancer Council of Western Australia
- Swedish Research Council [Radsprofessor 2015-0418]
- Knut and Alice Wallenberg Foundation
- Australian Genome Research Facility (AGRF)
- Alexander von Humboldt Foundation
- Australian Postgraduate Awards (APA) Scholarship
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The expression of the compact mammalian mitochondrial genome requires transcription, RNA processing, translation and RNA decay, much like the more complex chromosomal systems, and here we use it as a model system to understand the fundamental aspects of gene expression. Here we combine RNase footprinting with PAR-CLIP at unprecedented depth to reveal the importance of RNA-protein interactions in dictating RNA folding within the mitochondrial transcriptome. We show that LRPPRC, in complex with its protein partner SLIRP, binds throughout the mitochondrial transcriptome, with a preference for mRNAs, and its loss affects the entire secondary structure and stability of the transcriptome. We demonstrate that the LRPPRC-SLIRP complex is a global RNA chaperone that stabilizes RNA structures to expose the required sites for translation, stabilization, and polyadenylation. Our findings reveal a general mechanism where extensive RNA-protein interactions ensure that RNA is accessible for its biological functions.
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