Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-017-00511-w
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Funding
- NUS
- Synthetic Biology Initiative of the National University of Singapore [DPRT/943/09/14]
- Summit Research Program of the National University Health System [NUHSRO/2016/053/SRP/05]
- Ministry of Education of Singapore [MOE/2014/T2/2/128]
- US Air Force [FA/2386/12/1/4055]
- U.S. Defense Threat Reduction Agency [HDTRA1-13-0037]
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Environmental pH is a fundamental signal continuously directing the metabolism and behavior of living cells. Programming the precise cellular response toward environmental pH is, therefore, crucial for engineering cells for increasingly sophisticated functions. Herein, we engineer a set of riboswitch-based pH-sensing genetic devices to enable the control of gene expression according to differential environmental pH. We next develop a digital pH-sensing system to utilize the analogue-sensing behavior of these devices for high-resolution recording of host cell exposure to discrete external pH levels. The application of this digital pH-sensing system is demonstrated in a genetic program that autonomously regulated the evolutionary engineering of host cells for improved tolerance to a broad spectrum of organic acids, a valuable phenotype for metabolic engineering and bioremediation applications.
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