Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 63, Issue 8, Pages 671-681Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/cjm-2017-0067
Keywords
mtDNA; mobile introns; protein purification; endonuclease assays.
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Faculty of Science Graduate Award program (University of Manitoba)
- University of Manitoba Faculty of Graduate Studies GETS program
- Islamic Development Bank Merit Scholarship Programme
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In vitro characterization of 3 LAGLIDADG-type homing endonucleases (HEs) ( I-CcaI, I-CcaII, and I-AstI) that belong to the I-OnuI family showed that they are functional HEs that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologues that have inserted into 3 different mitochondrial genes: rns, rnl, and cox3. The endonuclease activity of I-CcaI was tested using various substrates, and its minimum DNA recognition sequence was estimated to be 26 nt. This set of HEs may provide some insight into how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of currently available HEs that may have various biotechnology applications.
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