Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-017-01141-y
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Funding
- National Natural Science Foundation of China [61675071, 61405062, 91233208]
- Pearl River Nova Program of Guangzhou [201710010010]
- Guangdong Innovative Research Team Program [201001D00104799318, 2011D039]
- Natural Science Foundation of Guangdong province [2014A030313445]
- China Postdoctoral Science Foundation [2016M600659, XJ2015018, 2014T70818]
- Joint International Research Laboratory of Optical Information
- Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province (Shenzhen University)
- Swedish Research Council [2015-00160, 2016-03804]
- Swedish Research Council [2015-00160, 2016-03804] Funding Source: Swedish Research Council
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Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light-matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution similar to 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution similar to 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.
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