Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-017-00230-2
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Funding
- National Institutes of Health (NIH) [1S10RR026549-01]
- National Hemophilia Foundation Judith Graham Pool Postdoctoral Research Fellowship
- National Science Foundation Graduate Research Fellowship [DGE-1144152]
- American Heart Association [13SDG17000054]
- NIH NIGMS [R35 GM119537]
- Wyss Institute
- NIH [HL108248, HL103526]
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Von Willebrand factor, an ultralarge concatemeric blood protein, must bind to platelet GPIb alpha during bleeding to mediate hemostasis, but not in the normal circulation to avoid thrombosis. Von Willebrand factor is proposed to be mechanically activated by flow, but the mechanism remains unclear. Using microfluidics with single-molecule imaging, we simultaneously monitored reversible Von Willebrand factor extension and binding to GPIb alpha under flow. We show that Von Willebrand factor is activated through a two-step conformational transition: first, elongation from compact to linear form, and subsequently, a tension-dependent local transition to a state with high affinity for GPIb alpha. High-affinity sites develop only in upstream regions of VWF where tension exceeds similar to 21 pN and depend upon electrostatic interactions. Re-compaction of Von Willebrand factor is accelerated by intramolecular interactions and increases GPIb alpha dissociation rate. This mechanism enables VWF to be locally activated by hydrodynamic force in hemorrhage and rapidly deactivated downstream, providing a paradigm for hierarchical mechano-regulation of receptor-ligand binding.
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