Journal
CHEMICAL ENGINEERING JOURNAL
Volume 322, Issue -, Pages 510-515Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2017.04.022
Keywords
Enzymes; Enzyme catalysis; Single enzyme caged nanoparticles; Enzyme stabilization; Enzyme immobilization
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Funding
- Global Research Laboratory Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [2014K1A1A2043032]
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For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called ion-pairing. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture. (C) 2017 Elsevier B.V. All rights reserved.
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