Journal
BIOSENSORS & BIOELECTRONICS
Volume 94, Issue -, Pages 694-700Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.03.064
Keywords
Potassium doped graphene; Aptamer; Tri infinite amplification; Matrix metalloproteinases-2; Immunoassay
Categories
Funding
- National Key Scientific Instrument and Equipment Development Project of China [21627809]
- National Natural Science Foundation of China [21375047, 21377046, 21405059, 21575050, 21505051]
- Special Foundation for Taishan Scholar Professorship of Shandong Province [ts20130937]
- China Postdoctoral Science Foundation [2016M592125]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Key Research and Development Program of Shandong Province [2015GGH301001]
- Technology Research Project of Shandong Provincial Education Department [J15LC07]
- Graduate Innovation Foundation of University of Jinan [YCXB15004]
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Herein, a super-labeled immunoassay was fabricated for matrix metalloproteinases-2 detection. A self-corrosion ITO micro circuit board was designed in this sensing platform to reduce the random error in the same testing condition, and the self-constructed sensing platform is portable with a cheap price. The K-modified graphene (K-GS) was utilized as the matrix material, which was synthesized well by phenylate and phenanthrene through the polar bond of nonpolar molecule phenylate and the 71-71 interaction for the first time. An aptamer-based labels based on Au nanoparticles (AuNPs), thionine (Th) and horseradish peroxidase (HRP) were applied as the signal source for tri infinite amplification. This fabricated super-labeled immunoassay exhibit excellent performance for MMPs-2 detection. It displayed a broad linear range of 10-4-10 ng/mL with a low detection limit of 35 fg/mL, which may have a potential application in the clinical diagnose.
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