Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms15261
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Funding
- Watkins Chair of Obstetrics and Gynecology
- National Institutes of Health National Center for Translational Research in Reproduction and Infertility (NCTRI) Center for Reproductive Health After Disease [P50HD076188]
- NCATS [UH3TR001207]
- NICHD [UH3TR001207]
- NIEHS [UH3TR001207]
- OWHR [UH3TR001207]
- NIH Common Fund [UH3TR001207]
- NIH [1K01DK099454-01]
- Burroughs Wellcome Fund
- NSF Graduate Research Fellowship Program [DGE-1324585]
- Eunice Kennedy Shriver NICHD/ NIH (NCTRI) Grant [P50-HD28934]
- NCI CCSG [P30 CA060553]
- Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF) [NNCI-1542205]
- MRSEC program (NSF) at the Materials Research Center [DMR-1121262]
- International Institute for Nanotechnology (IIN)
- Keck Foundation
- State of Illinois through IIN
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Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects the survival of ovarian follicles. 30 degrees and 60 degrees scaffolds provide corners that surround follicles on multiple sides while 90 degrees scaffolds have an open porosity that limits follicle-scaffold interaction. As the amount of scaffold interaction increases, follicle spreading is limited and survival increases. Follicle-seeded scaffolds become highly vascularized and ovarian function is fully restored when implanted in surgically sterilized mice. Moreover, pups are born through natural mating and thrive through maternal lactation. These findings present an in vivo functional ovarian implant designed with 3D printing, and indicate that scaffold pore architecture is a critical variable in additively manufactured scaffold design for functional tissue engineering.
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