Journal
NATURE COMMUNICATIONS
Volume 8, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-017-01325-6
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Funding
- UQ Postdoctoral Fellowship
- NHMRC/National Heart Foundation Career Development Fellowship [1083811]
- HMRC Research Fellowship [1044041]
- ARC Discovery Project grant [DP150104119]
- NHMRC [1067405, 1037320]
- ARC LIEF [LE130100078]
- ACRF
- National Health and Medical Research Council of Australia [1083811, 1067405] Funding Source: NHMRC
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Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
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