Journal
BIO-PROTOCOL
Volume 7, Issue 16, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2520
Keywords
Escherichia coli; Enterobacteria; Polyketide; Colibactin; Genotoxin; DNA damage; Cell culture; Infection
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Funding
- French Institut National du Cancer [INCAPLBIO13-123]
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Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death. Here we describe a simple method to demonstrate the genotoxicity of bacteria producing colibactin following a short infection of cultured mammalian cells with pks(+) E. coli.
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