Journal
ONCOLOGY LETTERS
Volume 14, Issue 3, Pages 2869-2877Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2017.6521
Keywords
ovarian cancer; near-infrared fluorescence imaging; metastatic cancer; nanoparticles; retroperitoneal lymph nodes
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Funding
- National Natural Science Foundation of China [81472423]
- Shanghai Natural Science Foundation of China [13ZR1404300]
- Experimental Animal Special Fund of Science and Technology Commission of Shanghai [13140901500]
- Chinese Natural Science Foundation project [81301261, 21374059]
- Key Project for Major Diseases of Health System of Shanghai [2013ZYJB0201]
- National Key Research and Development Program [2016YFC1303100, 2016YFC1303101, 2016YFC1303102, 2016YFC1303103]
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Lymph node metastasis occurs in early-stage and late-stage ovarian cancers. Systematic lymphadenectomy is frequently conducted in an attempt to prevent disease progression. However, this method is associated with multiple complications. Therefore, it is necessary to develop a less invasive and more sensitive method for detecting lymphatic metastasis in ovarian cancer. The aim of the present study was to develop an appropriate fluorescent label for the analysis of lymphatic metastasis in vivo. To this end, epithelial ovarian cancer cells with high potential for lymph node metastasis were labeled using mCherry fluorescence. The cells were then imaged in vitro to determine the expression of mCherry, and in a mouse xenograft model in vivo. The data demonstrated the successful identification of metastatic retroperitoneal lymph nodes by co-localization with lymph nodes labeled by near-infrared fluorescence nanoparticles in vivo. These data provided important insights into the further development of methods for intra-operative identification of lymphatic metastasis and the mechanisms underlying lymphatic metastasis.
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