4.7 Article

Understanding the thermal stability of human serum proteins with the related near-infrared spectral variables selected by Monte Carlo-uninformative variable elimination

Journal

CHINESE CHEMICAL LETTERS
Volume 28, Issue 7, Pages 1447-1452

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2017.03.021

Keywords

Near-infrared spectroscopy; Temperature dependent spectroscopy; Monte Carlo-uninformative variable; elimination; Protein; Human serum

Funding

  1. National Natural Science Foundation of China [21475068]

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Understanding the thermal stability of the proteins in human serum is essential since human serum is the important source of pharmaceutical proteins. Near-infrared (NIR) spectroscopy was applied to the investigation of thermal changes in secondary structure and hydration of human serum proteins. However, as a multicomponent system, the overlap of the broad NIR bands makes the structural analysis very difficult directly using the spectra of serum samples. Therefore, continuous wavelet transform (CWT) was used to improve the resolution of NIR spectra, and Monte Carlo-uninformative variable elimination (MC-UVE) method was applied to the selection of the variables associated with the proteins for the structural analysis. The variables (5956, 5867, 5815, 5747, 4525, 4401, 4359 and 4328 cm(-1)) related to protein secondary structures and those (7074, 6951, 6827 and 6700 cm(-1)) connected with water species were selected. Then, the thermal stability was analyzed through the intensity variations of the selected variables with temperature from 30 degrees C to 80 degrees C. It was found that the variation of the spectral variables related to both alpha-helix and,beta-sheet changes apparently around 60 degrees C, indicating the beginning of the thermal denaturation and the transition from alpha-helix to beta-sheet. Moreover, an obvious change was found around 60 degrees C for the content of the water specie S-3, i.e., the water cluster containing three hydrogen bonds. The result demonstrates that MC-UVE can identify the protein-related NIR spectral variables, and the water species may be a marker for investigation of the structural change of proteins in biochemical systems. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

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