Journal
ONCOLOGY LETTERS
Volume 15, Issue 2, Pages 2006-2009Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2017.7465
Keywords
3D spheroids; oxygen absorber; anoxia; microtissue storage
Categories
Funding
- TRI-Genotoul
- ITAV imaging facility
- University of Toulouse
- CNRS
- Agence Nationale de la Recherche ANR
- la Ligue Contre le Cancer
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Multicellular tumor spheroids models are of increasing interest in preclinical studies and pharmacological evaluation. However, their storage and transport is often a limitation because it requires adapted and expensive procedures. Here, we propose a very simple method to store 3D spheroids, using a procedure based on oxygen absorber-induced anoxia. We report that oxygen absorbers allow generating an anoxic environment for spheroid storage in culture plates. Oxygen absorber-induced anoxia fully and reversibly arrests spheroid growth for 4 days at 37 degrees C and up to 18 days at 4 degrees C. We then show that the response to etoposide is comparable in spheroids preserved in conditions of absorber-induced anoxia at 4 degrees C and spheroids kept in normoxia at 37 degrees C. These results represent a major improvement that should simplify the storage, transport and use of 3D spheroids.
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