Journal
ONCOLOGY LETTERS
Volume 14, Issue 3, Pages 3594-3600Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2017.6638
Keywords
Salvia miltiorrhiza extract; human matrix metalloproteinase-9; invasion; activator protein 1; breast cancer MCF-7 cell line
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Funding
- National Research Foundation of Korea (NRF) - Nuclear Research & Development Program of the National Research Foundation [NRF-2012M2A2A6011335]
- Korean government (the Ministry of Education, Science Technology), Republic of Korea [2011-0030130]
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Cancer cell invasion is crucial for metastasis. A major factor in the capacity of cancer cell invasion is the activation of matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix. Salvia miltiorrhiza has been used as a promotion for blood circulation to remove blood stasis. Numerous previous studies have demonstrated that S. miltiorrhiza extracts (SME) decrease lipid levels and inhibit inflammation. However, the mechanism behind the effect of SME on breast cancer invasion has not been identified. The inhibitory effects of SME on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 expression were assessed using western blotting, reverse transcription-quantitative polymerase chain reaction and zymography assays. MMP-9 upstream signal proteins, including mitogen-activated protein kinases and activator protein 1 (AP-1) were also investigated. Cell invasion was assessed using a matrigel invasion assay. The present study demonstrated the inhibitory effects of the SME ethanol solution on MMP-9 expression and cell invasion in TPA-treated MCF-7 breast cancer cells. SME suppressed TPA -induced MMP-9 expression and MCF-7 cell invasion by blocking the transcriptional activation of AP-1. SME may possess therapeutic potential for inhibiting breast cancer cell invasiveness.
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