Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 33, Pages 27504-27511Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b07684
Keywords
affinity sensing aptamer; G-quadruplex; graphene field-effect transistor (GFET); insulin
Funding
- National Institutes of Health [1DP3DK101085-01, 1R33CA196470-01A1]
- National Science Foundation [ECCS-1509760]
- China Scholarship Council [201506120133]
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This paper presents an approach to the real-time, label-free, specific, and sensitive monitoring of insulin using a graphene aptameric nanosensor. The nanosensor is configured as a field-effect transistor, whose graphene-based conducting channel is functionalized with a guanine-rich IGA3 aptamer. The negatively charged aptamer folds into a compact and stable antiparallel or parallel G-quadruplex conformation upon binding with insulin, resulting in a change in the carrier density, and hence the electrical conductance, of the graphene. The change in the electrical conductance is then measured to enable the real-time monitoring of insulin levels. Testing has shown that the nanosensor offers an estimated limit of detection down to 35 pM and is functional in Krebs Ringer bicarbonate buffer, a standard pancreatic islet perfusion medium. These results demonstrate the potential utility of this approach in label-free monitoring of insulin and in timely prediction of accurate insulin dosage in clinical diagnostics.
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