Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 14, Issue 2, Pages 1327-1336Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2017.4701
Keywords
indigo naturalis; Chinese herbal medicine; ulcerative colitis; inflammation; colonic mucosal changes
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Funding
- G20 Project Support, The Beijing Municipal Science and Technology Plan (Beijing municipal Science And Technology Commission, Beijing, China) [Z151100003815011]
- Youth Fund of National Science Foundation of China (State Natural Science Funds Commission, Beijing, China) [81403369]
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The effects of indigo naturalis (IN), which is a traditional Chinese herbal formulation, have been clinically demonstrated in treating refractory ulcerative colitis (UC). The present study aimed to verify the effects and mechanisms of IN in experimental UC rats. A total of 48 male Sprague-Dawley rats were randomly divided into six groups: Chow, model, high-dose IN, medium-dose IN, low-dose IN and mesalazine (a bowel-specific aminosalicylate drug) groups. The models were administered 3.5% dextran sodium sulphate solution for 7 days. The treatment groups were administered IN or mesalazine and then sacrificed and sampled on day 8. Disease activity index (DAI), histological damage score (HDS) and myeloperoxidase (MPO) activity were used to evaluate the severity of UC. Colon and serum cytokines were detected using liquid-phase chip technology and the expression of occludin protein in colonic mucosa was assessed by immunohistochemistry and western blot analysis. The results indicated that the oral administration of IN may reduce DAI, HDS and MPO activity. IN also reduced the expression of inflammatory cytokines and increased the expression of colonic mucosal repair-related cytokines and occludin protein. These results highlight the potential of IN as a therapeutic agent for treating UC through its action of inflammation control and colonic mucosal damage repair.
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