Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1858, Issue 9, Pages 733-741Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2017.03.007
Keywords
Geobacter sulfurreducens; Outer membrane cytochrome; NMR; Redox protein; Solution structure
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Funding
- Fundacao para a Ciencia e a Tecnologia (Portugal) [SFRH/BD/61952/2009, UID/Multi/04378/2013]
- Ministerio de Economia y Competitividad (Spain) [CTQ2014-52633-P]
- Fundacao para a Ciencia e a Tecnologia [RECI/BBB-BQB/0230/2012]
- [PTDC/BBB-BQB/3554/2014]
- [SFRH/BD/89701/2012]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/89701/2012, SFRH/BD/61952/2009] Funding Source: FCT
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Gene knock-out studies on Geobacter sulfurreducens cells showed that the outer membrane-associated monoheme cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI). In addition, microarray analysis of an OmcF-deficient mutant revealed that many of the genes with decreased transcript level were those whose expression is up-regulated in cells grown with a graphite electrode as electron acceptor, suggesting that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electricity production by G. sulfurreducens in microbial fuel cells. N-15,C-13-labeled OmcF was produced and NMR spectroscopy was used to determine the solution structure of the protein in the fully reduced state and the pH dependent conformational changes. In addition, 15N relaxation NMR experiments were used to characterize the overall and internal backbone dynamics of OmcF. The structure obtained is well-defined, with an average pairwise root mean square deviation of 0.37 angstrom for the backbone atoms and 0.98 angstrom for all heavy atoms. For the first time a solution structure and the protein motions were determined for an outer membrane cytochrome from G. sulfurreducens, which constitutes an important step to understand the extracellular electron transfer mechanism in Geobacter cells.
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