Journal
ANALYTICAL BIOCHEMISTRY
Volume 534, Issue -, Pages 10-13Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2017.07.010
Keywords
Biolayer interferometry; ELISA; Malaria; Histidine-rich protein 2; Binding kinetics
Funding
- Bill and Melinda Gates Foundation [OPP1135840]
- PATH: Diagnostics for malaria elimination toward eradication [1758-00-06-00]
- National Science Foundation Graduate Research Fellowship Program [1445197]
- Bill and Melinda Gates Foundation [OPP1135840] Funding Source: Bill and Melinda Gates Foundation
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Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checker-board ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format. (C) 2017 The Authors. Published by Elsevier Inc.
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