Journal
SAUDI PHARMACEUTICAL JOURNAL
Volume 25, Issue 4, Pages 633-637Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jsps.2017.04.036
Keywords
Lycorine hydrochloride; MCF-7; Death receptor pathway; Apoptosis
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Funding
- Postdoctoral Foundation of China [2015M581467]
- Natural Science Foundation of Heilongjiang Province [D201138]
- Postdoctoral Foundation of Hei Long Jiang Province [LBH-Z15107]
- Science and Technology Innovation Team Program in Higher Education Institutions of Heilongjiang Province [2014TD009]
- Harbin Special Foundation [2013RFQXJ150]
- Training Program for Young Innovative Talented Person of Heilongjiang Province [UNPYSCT-2015071]
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As research was conducted on the early apoptosis of human breast cancer cell MCF-7 caused by lycorine hydrochloride and the expression of the related apoptosis proteins. The early-period apoptosis rate of human breast cancer cell MCF-7 was tested with the AnnexinV/PI double staining and flow cytometry. The Western Blotting method was also used to detect the protein expression conditions of Fas, FasL, Caspase-8 and Bid. The results showed that the higher the dose, the higher the rate of apoptosis and that the rate of apoptosis was dependent on the dose; the relative protein activity of Fas, FasL, Caspase-8 and bid gradually rose with the increase of lycorine dosage and the activities revealed certain dose-independence. Results showed that lycorine hydrochloride could induce the apoptosis of human breast cancer cell MCF-7 through the death receptor pathway. (C) 2017 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
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