Journal
INDIAN JOURNAL OF BIOTECHNOLOGY
Volume 16, Issue 1, Pages 133-137Publisher
NATL INST SCIENCE COMMUNICATION-NISCAIR
Keywords
Adjuvants; in vitro propagation; MS medium; Tinospora cordifolia
Categories
Ask authors/readers for more resources
An efficient plant regeneration protocol was developed through direct organogenesis in T. cordifoila, a National Medicinal Plant Board (NMPB) prioritized medicinal plant. Mature in vivo nodal explants were used for optimal development of multiple shoots in vitro. Maximum number of shoots per nodal explant (7.9 +/- 0.45) with the highest shoot length (9.3 +/- 0.48 cm) was developed on MS medium supplemented with N6-benzyladenine (4. 44 mu M) in combination with N6-2-iso-pentenyl adenine (2.45 mu M). During the shoot multiplication, leaching of phenolics was observed and to avoid this, different adjuvants (activated charcoal, polyvinyl pyrrolidone and ascorbic acid) were used. Polyvinyl pyrrolidone at 1.0% in combination with optimized hormones when incorporated in MS medium, phenolic secretion was controlled successfully. The highest % response of rooting (89%) with 8.3 +/- 0.46 cm root length was observed on 1/2 strength MS medium augmented with indole butyric acid (2.45 mu M). Complete plantlets were transferred to earthen pots containing a mixture of autoclaved garden soil and cocopeat (2:1) and then shifted to the culture chamber for further hardening and acclimatization. Plants after acclimatization survived well in nature.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available