Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 34, Pages 28331-28338Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b09848
Keywords
tetraphenylethene; aggregation-induced emission; linear discrimination analysis; bacterial imaging; bacterial discrimination
Funding
- National Natural Science Foundation of China [21272027]
- National Key Research and Development Program of China [2016YFC1202502]
- National High Technology Research and Development Program of China (863 Program) [2013AA032205]
- Beijing Nova Program of China [Z151100000315086]
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Imaging-based total bacterial count and type identification of bacteria play crucial roles in clinical diagnostics; public health, biological and medical science, and environmental protection. Herein, we designed and synthesized a series of tetraphenylethenes (TPEs) functionalized with one or two aldehyde, carboxylic acid, and quaternary ammonium groups, which were successfully used as fluorescent materials for rapid and efficient staining of eight kinds of reptesentative bacterial species, including pathogenic bacteria Vibrio cholera, Klebsiella pneumoniae, and Listeria monocytogenes and potential bioterrorism agent Yersinia pestis. By comparing the fluorescence intensity changes of the aggregation-induced-erinssion (AIE) materials before and after bacteria incubation, the sensing mechanisms (electrostatic versus hydrophobic interactions) were simply discussed. Moreover, the designed AIE materials were successfully used as an efficient artificial tongue for bacteria discrimination, and all of the bacteria tested were identified via linear discriminant analysis. Our current work provided a general method for simultaneous broad-spectrum bacterial imaging and species discrimination, which is helpful for bacteria surveillance in many fields.
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