Journal
EMBO JOURNAL
Volume 36, Issue 17, Pages 2553-2566Publisher
WILEY
DOI: 10.15252/embj.201796883
Keywords
centriole; dendritic transport; dynein; neuronal ciliogenesis
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Funding
- National Basic Research Program of China [2017YFA0503501, 2017YFA0102900, 2013CB945602]
- National Natural Science Foundation of China [31190063, 31301134]
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Neuronal cilia that are formed at the dendritic endings of sensory neurons are essential for sensory perception. However, it remains unclear how the centriole-derived basal body is positioned to form a template for cilium formation. Using fluorescence time-lapse microscopy, we show that the centriole translocates from the cell body to the dendrite tip in the Caenorhabditis elegans sensory neurons. The centriolar protein SAS-5 interacts with the dynein light-chain LC8 and conditional mutations of cytoplasmic dynein-1 block centriole translocation and ciliogenesis. The components of the central tube are essential for the biogenesis of centrioles, which later drive ciliogenesis in the dendrite; however, the centriole loses these components at the late stage of centriole translocation and subsequently recruits transition zone and intraflagellar transport proteins. Together, our results provide a comprehensive model of ciliogenesis in sensory neurons and reveal the importance of the dynein-dependent centriole translocation in this process.
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