Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 82, Issue -, Pages 1-5Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2017.07.011
Keywords
Beta-lactam antibiotic monitoring; Minimally invasive; Continuous monitoring; Antibiotic resistance
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Funding
- National Institutes of Health Research (NIHR) [II-LA-0214-20008] Funding Source: National Institutes of Health Research (NIHR)
- National Institute for Health Research [II-LA-0214-20008] Funding Source: researchfish
- Department of Health [II-LA-0214-20008] Funding Source: Medline
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Antimicrobial resistance is a leading patient safety issue. There is a need to develop novel mechanisms for monitoring and subsequently improving the precision of how we use antibiotics. A surface modified microneedle array was developed for monitoring beta-lactam antibiotic levels in human interstitial fluid. The sensor was fabricated by anodically electrodepositing iridium oxide (AEIROF) onto a platinum surface on the microneedle followed by fixation of beta-lactamase enzyme within a hydrogel. Calibration of the sensor was performed to penicillin-G in buffer solution (PBS) and artificial interstitial fluid (ISF). Further calibration of a platinum disc electrode was undertaken using amoxicillin and ceftriaxone. Open-circuit potentials were performed and data analysed using the Hill equation and log(concentration [M]) plots. The microneedle sensor demonstrated high reproducibility between penicillin-G runs in PBS with mean Km (+/- 1SD) = 0.0044 +/- 0.0013 M and mean slope function of log(concentration plots) 29 +/- 1.80 mV/decade (r(2) = 0.933). Response was reproducible after 28 days storage at 4 degrees C. In artificial ISF, the sensors response was Km (+/- 1SD) = 0.0077 +/- 0.0187 M and a slope function of 34 +/- 1.85 mv/decade (r(2) = 0.995). Our results suggest that microneedle array based beta-lactam sensing may be a future application of this AEIROF based enzymatic sensor.
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