Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 32, Issue -, Pages 112-122Publisher
ELSEVIER
DOI: 10.1016/j.jff.2017.02.026
Keywords
Serine metalloprotease; Lactococcus lactis subsp lactis BR16; Bovine casein; Adsorption/desorption; Antibacterial peptide
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Funding
- Ministry of Higher Education and Scientific Research of Algeria and Cooperation program Franco Algerian Hubert Curien Partnership (PHC) Tassili
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An extracellular serine metalloprotease produced by Lactococcus lactis subsp lactis BR16 isolated from Algerian traditional fermented food was used for hydrolyzing bovine casein in order to identify antibacterial peptide. Antibacterial peptide was separated from peptic hydrolysates by adsorption/desorption then analyzed using reverse phase high performance liquid chromatography (RP-HPLC) and active fraction was analyzed by MALDI-TOF mass spectrometry. A new antibacterial peptide was identified as SSSEESII from the subunit alpha(s2) of casein corresponding of the fragment (24-31). This peptide demonstrated antibacterial activity against two Gram-positive bacteria: Listeria innocua ATCC 33090 and Micrococcus luteus ATCC 4698, and two Gram-negative bacteria: Escherichia coli ATCC 25922 and Salmonella enteritidis ATCC13076. Prediction of peptide secondary structure indicated that this peptide is anionic and must have random coil structure with a small region of hydrophobicity, which confers to the peptide unusual behavior compared to conventional antimicrobial peptides. (C) 2017 Elsevier Ltd. All rights reserved.
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