Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 29, Issue -, Pages 275-280Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2016.12.035
Keywords
Phenyl-gamma-valerolactone; Flavan-3-ol metabolite; UPEC; Peripheral metabolism; Cranberry; Urinary tract infections
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Funding
- Fundacion Seneca (Murcia Region, Spain)
- Spanish MINECO [AGL2012-40172-C02-01]
- COST Action - COST (European Cooperation in Science and Technology) [FA 1403]
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Urinary tract infections (UTI) are mostly caused by uropathogenic Escherichia coli (UPEC). Cranberry based products have shown preventive effects against UTI, and this has been partially attributed to their A-type proanthocyanidin content. However, recent evidence reports phenyl-gamma-valerolactones as the most relevant urinary metabolites of cranberry procyanidins, and candidates these compounds as plausible responsible for the protective effects of cranberries against UTI. This paper studied the inhibition of the adherence of UPEC ATCC (R) 53503 (TM) to T24 bladder epithelial cells by physiological concentrations of differently sulphated dihydroxyphenyl-gamma-valerolactones. Moreover, the transformations of these molecules in the cell media were evaluated by UHPLC-MSn. All dihydroxyphenyl-gamma-valerolactone derivatives showed anti-adhesive activity at 100 mu M, while 5-(3'-hydroxypheny1)-gamma-valerolactone-4-O-sulphate also showed neuro-protective effects at 50 mu M. Some compounds underwent extensive metabolism during cell incubation, mainly deconjugation of sulphate moieties and opening of the lactone ring. These results shed light on the flavan-3-ol metabolites behind the prophylactic effect of cranberries against UTI. (C) 2016 Elsevier Ltd. All rights reserved.
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