Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 28, Issue -, Pages 48-58Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2016.11.021
Keywords
Palm kernel expeller glutelin-2; Peptides; Angiotensin-I converting enzyme; Spontaneously hypertensive rats; Vascular endothelial cells; Endothelin-1
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Funding
- program of Science and Technology of 12th Five-year Plan [2012BAD31B03]
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Palm kernel expeller glutelin-2 hydrolysates (PKEGH) with high ACE-inhibitory activity (80.24%) were obtained by the sequential digestion with alcalase, flavourzyme, pepsin and trypsin assisted by high pressure pretreatment. PKEGH were separated by ultrafiltration, Sephadex G-25 gel chromatography and RP-HPLC. Finally four novel ACE-inhibitory peptides (Ala-Asp-Val-Phe-Asn-Pro-Arg, Val-Val-Leu-Tyr-Lys, Leu-Pro-Ile-Leu-Arg and Val-Ile-Glu-Pro-Arg) were identified, of which Val-Val-Leu-Tyr-Lys showed the highest activity (IC50: 533.9 mu M). All the four peptides exhibited potent non-competitive ACE inhibition and relatively good stability against gastrointestinal enzymes digestion. Moreover, these peptides significantly lowered the endothelin-1 content in EA.hy926 cells without significant cytotoxicity, and protected vascular endothelial cells from reactive oxygen species mediated damage. Furthermore, the PKEGH and individual ACE-inhibitory peptides identified from it showed chronic antihypertensive effects in spontaneously hypertensive rats after several days weeks administration. Result showed that Palm kernel expeller glutelin-2 could be effectively converted to produce ACE-inhibitory or antihypertensive peptides. (C) 2016 Elsevier Ltd. All rights reserved.
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