Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 32, Issue -, Pages 123-130Publisher
ELSEVIER
DOI: 10.1016/j.jff.2017.02.018
Keywords
Luteolin; RAW264.7 macrophage; Inflammatory response; STAT3
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Funding
- Scientific Planning Project of the Applied Basic Research of Jiangsu Province by the China government [BK2013103]
- National Natural Science Foundation of China by the China government [81200577]
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Luteolin (Lut), a natural flavonoid extracted from plants, possesses several beneficial bioactivities. However, little is known about its anti-inflammatory properties. This study was designed to investigate the anti-inflammatory effects and underlying molecular mechanisms of luteolin in LPS plus IFN-gamma activated RAW264.7 macrophages (M1 macrophages). RAW264.7 cells were induced to M1 polarization by LPS plus IFN-gamma, accompanied with luteolin at non-cytotoxic doses. The results showed that the M1 macrophages presented dendrites characteristic with synapsis. In addition, luteolin can inhibit inflammation by modulating the expression of mediators, such as iNOS, IL-1 beta, IL-6, TNF-alpha, and CD86, whose expression was increased remarkably in the activated RAW264.7 cells. More importantly, the proteins p-STAT3-ser(727) and p-STAT3-tyr(705), which involved in inflammatory pathways, exhibited a dose-dependent degradation in M1 macrophages after treatment with luteolin. In conclusion, luteolin have potential applications as a functional food component in regulating inflammatory responses. (C) 2017 Elsevier Ltd. All rights reserved.
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