Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 31, Issue -, Pages 97-103Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2017.01.023
Keywords
Diabetes; HIF1 alpha; Lutein; Retinal angiogenesis; VEGF; XBP1
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Funding
- Department of Biotechnology, Government of India, New Delhi, India
- Council of Scientific and Industrial Research, New Delhi, India [BSC 0404, 9/1/BS/CFTRI(4)/2012-13-PPD]
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Lutein, a retinal-specific carotenoid, was assessed for angioprotective efficacy via hypoxia inducible factor (HIFI alpha) and X-box binding protein 1 (XBP1) pathway in diabetic retina. Streptozotocin-induced diabetic rats were orally gavaged with or without lutein (39 nmoliday) for 8 weeks. Lutein mediated regulation of vascular endothelial growth factor (VEGF) and superoxide dismutase 2 (SOD2) was analyzed using qPCR and immunoblotting. VEGF transcription factors, Hifl alpha and XBP1, in retina of streptozotocin induced diabetic rats were assessed using qPCR. Retinal protein carbonyl and lipid peroxide levels were quantified and transverse section of retina was stained for histopathology. Results indicate that lutein prevented the upregulation of VEGF and downregulation of SOD2 in retina of diabetic rats and also prevented abnormalities in ganglion cell, inner and outer nuclear layer in diabetic retina. This study suggests the anti VEGF property of lutein is perhaps via decrease in the mRNA expression of Hif1 alpha and Xbp1. (C) 2017 Elsevier Ltd. All rights reserved.
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