Isolation, antioxidant property and protective effect on PC12 cell of the main anthocyanin in fruit of Lycium ruthenicum Murray

The fruit of Lycium ruthenicum Murray has been widely used as a precious Chinese herbal medicine. In this study, the major anthocyanin in L ruthenicum fruit was obtained via an AKTA purifier system with YMC-Pack ODS-A column and identified by mass and nuclear magnetic resonance spectroscopy to be petunidin 3-0-[6-0-(4-0-(trans-p-coumaroyl)-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside]-5-0-[beta-D-glucopyranoside] (compound 1). Compound 1 exhibited higher scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 1,1-dipheny1-2-picrylhydra-zyl and superoxide radicals than the crude extract of anthocyanins. Moreover, compound 1 was demonstrated to have potential cyto-protective effect on hydrogen peroxide-induced intracellular reactive oxygen damage in neuronlike rat pheochromocytoma line cells by promoting cell proliferation, mitigating lipid peroxidation and regulating endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase). (C) 2017 Elsevier Ltd. All rights reserved.