Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 39, Issue -, Pages 37-43Publisher
ELSEVIER
DOI: 10.1016/j.jff.2017.09.058
Keywords
Ferulic acid; Inflammation; Caco-2 monolayer; Nejayote; Permeability
Categories
Funding
- Research Chair NutriOmics from Tecnologico de Monterrey
- Consejo Nacional de Ciencia y Tecnologia (CONACYT) [CVU 637952, 168705]
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The aim of this study was to evaluate in vitro the anti-inflammatory activity of phenolic acids absorbed through a Caco-2 monolayer after simulated digestion of spray-dried nejayote powder. Mouse macrophages (Raw 264.7) were treated with the basolateral fractions obtained at 2, 4, 8, or 12 h from the Caco-2 monolayer permeability experiment to measure the effects on NF-kappa B, TNF-alpha, COX-2, interleukin-10 (IL-10), and nitric oxide (NO) levels. When the digested samples were exposed to a Caco-2 monolayer, 67% of total ferulic acid (FA) present in spray-dried nejayote powder was recovered in the basolateral fractions after 8 h. Phenolic acids recovered after 2 h exerted the highest anti-inflammatory activity by inhibiting 53.8%, 93.4%, 86.3% and 84.7% the production of NF-kappa B, TNF-alpha, COX-2, and NO, respectively. Future in vivo studies should be designed to reach less than 7 mu M of FA and its derivatives to assess the potential health benefits. (C) 2017 Elsevier Ltd. All rights reserved.
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