Journal
ONCOTARGETS AND THERAPY
Volume 10, Issue -, Pages 1269-1278Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S125172
Keywords
colorectal cancer; miR-194; epithelial mesenchymal transition; migration; invasion
Categories
Ask authors/readers for more resources
MicroRNAs (miRNAs), as key regulators of gene expression, are closely related to tumor occurrence and progression. MiR-194 has been proved as a tumor regulatory factor in various cancers; however, the biological function and mechanism of action in colorectal cancer (CRC) have not been well explored. In the present study, we found that miR-194 expression is upregulated in CRC clinical specimens, while overexpression of miR-194 promotes cell migration and invasion in CRC cell lines. Besides, miR-194 significantly influenced the epithelialmesenchymal transition (EMT) markers by downregulating E-cadherin expression (P, 0.01) and upregulating vimentin and MMP-2 expression (P, 0.001, P, 0.05). Cell migration is the cell movement related to actin cytoskeleton. In this study, we found miR-194 increased cell polarization in SW480 cells. Moreover, zymography assay showed that miR-194 significantly upregulated the gelatin-degrading activity of MMP-2 (P, 0.01). Collectively, our findings suggest that miR-194 functions as a tumor promoter in CRC, which may provide new insights for the study of CRC development and metastasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available