Journal
BIOANALYSIS
Volume 9, Issue 17, Pages 1319-1328Publisher
FUTURE SCI LTD
DOI: 10.4155/bio-2017-0125
Keywords
LC-MS; lipopeptides; monolithic column; prolactin-releasing peptide; stability
Funding
- OPPC [CZ.2.16/3.1.00/21537]
- NPU I [LO1601]
- Academy of Sciences of the Czech Republic [RVO: 61388963]
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Aim: Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. Methodology & results: An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml(-1) in rat and macaque plasma samples. Conclusion: A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.
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