Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 68, Pages 9474-9477Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc05236g
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Funding
- Purdue University
- Ralph W. and Grace M. Showalter Research Trust
- Purdue University Center for Cancer Research's Jim and Diann Robbers Cancer Research Grant
- National Institutes of Health [P30 CA02318]
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Here, we describe an immunoassay approach for the detection of enzyme activity by quantitative PCR (qPCR) or parallel DNA sequencing which relies on activity-based probes linked to barcoding DNAs. We demonstrate this technique in the detection of serine hydrolase activities using a fluorophosphonate-oligonucleotide conjugate.
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