Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 17, Pages 9292-9298Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b02073
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Funding
- Natural Science Foundation of China [21475018, 21235001]
- Fundamental Research Funds for the Central Universities [N160504010, N141008001]
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A synthetic DNA machine performs quasi mechanical movements in response to external intervention, suggesting the promise of constructing sensitive and specific biosensors. Herein, a smart DNA walker biosensor for label free detection of carcinoembryonic antigen (CEA) is developed for the first time by a novel cascade amplification strategy of exonuclease (Exo) III -assisted target recycling amplification (ERA) and DNA walker. ERA as the first stage of amplification generates the walker DNA, while the autonomous traveling of the walker DNA on the substrate-modified silica microspheres as the second stage of amplification produces an ultrasensitive fluorescent signal with the help of N-methylmesoporphyrin IX (NMM). The DNA machine as a biosensor could be applied for transducing and quantifying signals from isothermal molecular amplifications, avoiding the complicated reporter elements and thermal cycling. The present biosensor achieves a detection limit of 1.2 pg.mL(-1) within a linear range of 10 pg.mL(-1) to 100 ng.mL(-1) for CEA, along with a favorable specificity. The practical applicability of the biosensor is demonstrated by the detection of CEA in human serum with satisfactory results; thus, it shows great potential in clinical diagnosis.
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