Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 17, Pages 8632-8637Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01282
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Funding
- Bill and Melinda Gates Foundation (GCE) [OPP1035881, OPP1097238]
- European Research Council (ERC Consolidator Grant) [615879]
- European Research Council (ERC) [615879] Funding Source: European Research Council (ERC)
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G-quadruplexes are nucleic acids structures stabilized by physiological concentration of potassium ions. Because low stability G-quadruplexes are hardly detectable by mass spectrometry, we optimized solvent conditions: isopropanol in a triethylamine/hexafluoroisopropanol mixture highly increased G-quadruplex sensitivity with no modification of the physiological G-quadruplex conformation. G-quadruplexes/G-quadruplex-ligand complexes were also correctly detected at concentration as low as 40 nM. Detection of the physiological conformation of G4s and their complexes opens up the possibility to perform high-throughput screening of G-quadruplex ligands for the development of drug molecules effective against critical human diseases.
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