Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 35, Pages 29481-29495Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b07066
Keywords
transfection; DNA; siRNA; mRNA; amphiphile; pyridinium
Funding
- NSF [CHE-0923077]
- Temple University Drug Discovery Initiative
- Temple University School of Pharmacy-Dean's Office
- Temple University Undergraduate Research Program
Ask authors/readers for more resources
This article presents the synthesis, self-assembly, and biological activity as transfection agents for pDNA, siRNA, and mRNA of novel pyridinium pseudogemini surfactants, interfacially engineered from the most efficient gemini surfactants and lipids generated in our amphiphile research program. Formulation of novel amphiphiles in water revealed supramolecular properties very similar to those of gemini surfactants, despite their lipidlike charge/mass ratio. This dual character was found also to enhance endosomal escape and, significantly increase the transfection efficiency. We were also successful in identifying the parameters governing the efficient delivery of pDNA, siRNA, and mRNA, drawing valuable structure-activity and structure-property relationships for each nucleic acid type, and establishing DNA/siRNA/mRNA comparisons. Several supramolecular complexes identified in this study proved to be extremely efficient nucleic acid delivery systems, displaying excellent serum stability and tissue penetration in three-dimensional organoids.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available