Journal
CHEMICAL SCIENCE
Volume 8, Issue 5, Pages 4051-4055Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7sc00789b
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Funding
- Swiss National Science Foundation [150073, 169451]
- Grants-in-Aid for Scientific Research [17H01211] Funding Source: KAKEN
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An antibacterial cyclic AS-48 protein was chemically synthesized by alpha-ketoacid-hydroxylamine (KAHA) ligation. Initial challenges associated with the exceptionally hydrophobic segments arising from the amphiphilic nature of the protein were resolved by the development of bespoke reaction conditions for hydrophobic segments, using hexafluoroisopropanol (HFIP) as a co-solvent. The synthetic protein displays similar biological activity and properties to those of the native protein. To support the current understanding of its antibacterial mode of action, we demonstrate the ability of AS-48 to be incorporated into synthetic multilamellar vesicles (MLVs).
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