Journal
CHEMICAL SCIENCE
Volume 8, Issue 2, Pages 1141-1145Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6sc03635j
Keywords
-
Categories
Funding
- New Faculty Startup Fund from the University of Nebraska-Lincoln
- National Institute of Health [1R01AI111862]
Ask authors/readers for more resources
We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels-Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a styrene-containing amino acid into proteins in response to amber nonsense codon. Fluorogenic labeling of purified proteins and intact proteins in live cells were demonstrated. The fluorogenicity of the styrene-tetrazine reaction can be potentially applied to the study of protein folding and function under physiological conditions with low background fluorescence interference.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available