4.8 Article

Multifunctional Yolk-Shell Nanostructure as a Superquencher for Fluorescent Analysis of Potassium Ion Using Guanine-Rich Oligonucleotides

Journal

ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 36, Pages 30406-30413

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b08732

Keywords

multifunctional yolk-shell nanoparticles; superquencher; fluorescent sensing; potassium ion; G-rich oligonucleotides

Funding

  1. National Key Research and Development Program of China [2016YFA0201204, 2016YFA0400902]
  2. National Program on Key Basic Research Project of China [2013CB933802]
  3. Shanghai Municipal Natural Science Foundation [17ZR1412100]
  4. Key Laboratory of Interfacial Physics and Technology, Chinese Academy of Sciences [CASKL-IPT1603]
  5. Startup Foundation for Doctors of Shanghai University of Engineering Science
  6. Youth Innovation Promotion Association, Chinese Academy of Sciences [2012205]
  7. Chinese Academy of Sciences
  8. National Natural Science Fund of China [21373260, 31470960, 51375294]
  9. King Saud University [RG-1436-005]

Ask authors/readers for more resources

The excellent performance of a biosensor generally depends on the high signal-to-noise ratio, and the superquencher plays a dominant role in fluorescent sensors. Novel nanoquenchers exhibited high quenching efficiency in various fluorescent assays of biological/chemical molecules. Here, we developed a novel nano-biosensor using Fe3O4@C yolk-shell nanoparticles (YSNPs) and studied their quenching effect. We found Fe3O4@C YSNP was a superquencher and exhibited an ultrastrong quenching ability, up to almost 100% quenching efficiency, toward fluorophores. Also, Fe3O4@C YSNPs possessed the most superior fluorescence restoration efficiency, due to biomolecular recognition event, compared to the other nanoquenchers, including bare Fe3O4 NPs, graphene oxide (GO), and single-wall carbon nanotubes (SWCNTs). On the basis of that, a fluorescent sensing platform for potassium-ion (K+) analysis with guanine (G)-rich oligonucleotides was designed. As a result, Fe3O4@C YSNP-based fluorescent sensors demonstrated excellent performance, with an ultrahigh sensitivity of a detection limit as low as 1.3 mu M, as well as a wide dynamic range from 50 mu M to 10 mM. The proposed method is fast, simple, and cost-effective, suggesting the great potential for practical applications in biomedical detection and clinical diagnosis.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available